Microbial Shelf Life Studies, Sanitation Audits, Environmental Surface Sampling, Air Microbial Testing and Biofeasibility Tests, Microbial and Biochemical Analytical Services, and Food Microbiology Services
About Us
Microbe Inotech Laboratories, Inc. was founded in 1991. Dr. Bruce C. Hemming started the company after ten years of progressively advanced experience at Monsanto Company. Microbe Inotech has taken the knowledge and experience of its key people and developed new methods in areas of environmental bioremediation, and analysis of materials in pharmaceuticals, foods and other bio-reactive materials. Our clients list includes many of the top 100 environmental engineering companies involved in major commercial and government bioremediation projects, major chemical and oil companies, other commercial, academic and government laboratories, microbial culture manufacturers and smaller remediation companies involved with leaking UST removals and remediation. We continue to service the needs of many food companies as well as other manufacturers in the chemical, pharmaceutical, nutraceutical business. We have served as expert witnesses in providing depositions, with resolutions prior to trial or testimony in federal courts involving cases with a microbiological component. The MiL performs proprietary microbial kinetic and endpoint assays. Our microbial databases contain profiles of over 3,000 species of microbes (including extensive E. coli 0157:H7 and yeast identification databases). We do microbial diagnostics research, have product development capabilities, and specialized skills in using automated, microbial characterization and identification tools and integrating results from combined Gas Chromatography Fatty Acid Methyl Ester (GC-FAME), RiboPrinter™ Molecular Typing, and Biolog® microplate reader instruments. We were selected (1996) by DuPont`s Qualicon™ subsidiary as one of their three, worldwide reference laboratories to assist in building and marketing their microbial, genetic characterizing, RiboPrinter`s™ databases and instruments. We offer real-time quantitative PCR detection and enumeration of bacteria and fungi. We are registered with the USDA and CDC as a Select Agent Laboratory capable of conducting research on the Anthrax causal agent, Bacillus anthracis. The MiL is able to use the significant experience of its key members and state of the art equipment to solve your problems involving microbial organisms and by-products.
Microbe Inotech Laboratories, Inc. was founded in 1991. Dr. Bruce C. Hemming started the company after ten years of progressively advanced experience at Monsanto Company.
Microbe Inotech has taken the knowledge and experience of its key people and developed new methods in areas of environmental bioremediation, and analysis of materials in pharmaceuticals, foods and other bio-reactive materials.
Our clients list includes many of the top 100 environmental engineering companies involved in major commercial and government bioremediation projects, major chemical and oil companies, other commercial, academic and government laboratories, microbial culture manufacturers and smaller remediation companies involved with leaking UST removals and remediation. We continue to service the needs of many food companies as well as other manufacturers in the chemical, pharmaceutical, nutraceutical business. We have served as expert witnesses in providing depositions, with resolutions prior to trial or testimony in federal courts involving cases with a microbiological component.
The MiL performs proprietary microbial kinetic and endpoint assays. Our microbial databases contain profiles of over 3,000 species of microbes (including extensive E. coli 0157:H7 and yeast identification databases).
We do microbial diagnostics research, have product development capabilities, and specialized skills in using automated, microbial characterization and identification tools and integrating results from combined Gas Chromatography Fatty Acid Methyl Ester (GC-FAME), RiboPrinter™ Molecular Typing, and Biolog® microplate reader instruments. We were selected (1996) by DuPont`s Qualicon™ subsidiary as one of their three, worldwide reference laboratories to assist in building and marketing their microbial, genetic characterizing, RiboPrinter`s™ databases and instruments. We offer real-time quantitative PCR detection and enumeration of bacteria and fungi. We are registered with the USDA and CDC as a Select Agent Laboratory capable of conducting research on the Anthrax causal agent, Bacillus anthracis.
The MiL is able to use the significant experience of its key members and state of the art equipment to solve your problems involving microbial organisms and by-products.
Microbiology Labs
Microbe Inotech Laboratories, Inc. are full service microbiology labs that can provide a broad range of testing services for the environmental, food, biotechnological and industrial communities. Our services include detection, isolation, enumeration, identification and confirmation of human and plant pathogenic organisms, industrial hygiene and medically related organisms, indicator and spoilage organisms. Our labs conduct challenge and microbial shelf life studies, sanitation audits, environmental surface sampling, air microbial testing, biotreatment, biodegradation, and biofeasibility tests. At the MiL, inc., our international base of clients are accustomed to very competitive pricing, quality service, objectivity, convenience, safety, reliable customer support, leading edge technology, and guaranteed turn-around time. Analyses Anthrax Screening Testing for Bacillus Anthracis We evaluate samples from state and local government agencies, police departments, as well as private companies and individuals. Current analysis includes Microscopic evaluation for rapid determination of biological agents if applicable quantitative real-time PCR detection and enumeration Selective plating of sample onto growth media that selects for Bacillus anthracis Confirmation identifications by 16S rRNA gene sequence analysis, the GC-FAME and/or Biolog methods of all bacteria that grow on the selective media over a 48 hour incubation period Samples may be sent in the form of environmental surface swabs or sponge wipes, as well as bulk powders, liquids or other materials Total testing time is currently 1-2 days, with a preliminary result available within the first 4 hours. Mold or Fungal Identification MicroSeqTM Fungal LSU rRNA Gene Sequencing (PE Applied Biosystems) DNA is extracted from fungal cells with enzymes. The LSU rRNA gene is selected and amplified using primers and thermalcyclers. The LSU rRNA gene product is sequenced using cycle sequencing chemistry. The reactions are analyzed using automated DNA sequencers and software. Unknown fungal samples are identified using the MicroSeqTM software and LSU rRNA database, containing over 1,500 entries. Either the entire LSU rRNA gene, or a smaller portion of the gene (300bp) can be sequenced. A printout of the sequence data and the identification is included in each report. GC-FAME (Gas Chromatograph Fatty Acid Methyl Ester) Also known as Cellular Fatty Acid Analysis, allows for microorganism identification based on the specific fatty acid composition of the cell wall. Fatty acids are extracted from cultured samples and are separated by gas chromatography. A computer generated, unique profile pattern of the extracted fatty acids (9 to 20 carbon chains long) are compared to our microbial databases of well over 2600 species and subspecies. Our reports include the fatty acid profiles, a listing of the best database matches, along with an assigned statistical probability value indicating the confidence level of the identifications. Fungal BIOLOG® This automated, identification system uses a 96-well microtiter plate with 95 different carbon sources. The microorganism of interest is applied to each well. Each microorganism has unique capacities to oxidize some of the various carbon sources. When these carbon sources are oxidized by the microorganism, a purple dye develops visible patterns of positive (purple) and negative (clear) wells which provide a metabolic signature of the organism. The system`s computer examines the pattern signature with its database to determine an identification. Databases include aerobic and anaerobic bacteria, yeasts and fungi. Biofeasibility Studies One of the most frequently requested set of tests is the Bioremediation Feasibility Series. It is a straight forward series of analyses that provide an objective evaluation of a site for bioremediation. The flow-chart shows the progression of tests as they are performed. The Sample can be soil, water, or sludge. When the sample is received at the laboratory, it is immediately prepared for the Total Heterotrophic Plate Count. Over the next 48 hours the Petri plates containing dilutions of the samples are incubated for optimum bacteria growth. The populations are counted twice, once at 24 hours and again at 48 hours. At the end of 48 hours, each bacterium has grown into a colony. Each colony is distinguishable by size, color and texture. It is at this time we determine, by colony morphology, the number of different types of bacteria in the soil or water. The next step is Isolation. Each type of bacterial colony is picked, using a sterile loop, and then streaked onto a new Petri plate. These plates are then incubated for 24 hours in order to produce sufficient quantities of the strain. The isolated strains of bacteria are then harvested for processing in the Endpoint Assay and for identification by GC-FAME and Biolog™. The Endpoint Assay tests the individual bacterial strains for their ability to use a specific substrate/contaminant as its only carbon source. This custom designed test uses a negative control of water and a positive control of a nutrient broth. The growth success of the bacteria is illustrated in a bar chart report. The Identification of the bacterial strains takes place while the Endpoint assay is running. Two methods are employed as a cross-check for precision and to provide an exact profile of the strain. The GC-FAME method provides a profile of the fatty acid composition of the bacteria and the Biolog™ method provides a characterization of the bacteria by the carbon sources the strain can consume. Next, the raw data is compiled and a summary Table of the bacterial identifications and Bar Charts of the Endpoint Assay raw data are created. These two items are incorporated in the full Report at the conclusion of all tests. The Bioremediation Feasibility Study is complete at this point. If either the client or the MiL provides nutrient Chemistry Results, we will integrate the results within the Bioremediation Feasibility Study in order to provide an Estimated Project Length report. This report estimates the time it would take your site to reach specified clean-up goals under ideal conditions. Other services include Characterization of Biodegrading Microorganisms Bioremediation Monitoring Microcosm Treatability Testing Wastewater Biotreatment Consulting Services
Microbe Inotech Laboratories, Inc. are full service microbiology labs that can provide a broad range of testing services for the environmental, food, biotechnological and industrial communities.
Our services include detection, isolation, enumeration, identification and confirmation of human and plant pathogenic organisms, industrial hygiene and medically related organisms, indicator and spoilage organisms.
Our labs conduct challenge and microbial shelf life studies, sanitation audits, environmental surface sampling, air microbial testing, biotreatment, biodegradation, and biofeasibility tests.
At the MiL, inc., our international base of clients are accustomed to very competitive pricing, quality service, objectivity, convenience, safety, reliable customer support, leading edge technology, and guaranteed turn-around time.
Anthrax Screening
Testing for Bacillus Anthracis
We evaluate samples from state and local government agencies, police departments, as well as private companies and individuals.
Current analysis includes
Total testing time is currently 1-2 days, with a preliminary result available within the first 4 hours.
Mold or Fungal Identification
MicroSeqTM Fungal LSU rRNA Gene Sequencing (PE Applied Biosystems)
DNA is extracted from fungal cells with enzymes. The LSU rRNA gene is selected and amplified using primers and thermalcyclers. The LSU rRNA gene product is sequenced using cycle sequencing chemistry. The reactions are analyzed using automated DNA sequencers and software. Unknown fungal samples are identified using the MicroSeqTM software and LSU rRNA database, containing over 1,500 entries. Either the entire LSU rRNA gene, or a smaller portion of the gene (300bp) can be sequenced. A printout of the sequence data and the identification is included in each report.
GC-FAME (Gas Chromatograph Fatty Acid Methyl Ester)
Also known as Cellular Fatty Acid Analysis, allows for microorganism identification based on the specific fatty acid composition of the cell wall. Fatty acids are extracted from cultured samples and are separated by gas chromatography. A computer generated, unique profile pattern of the extracted fatty acids (9 to 20 carbon chains long) are compared to our microbial databases of well over 2600 species and subspecies. Our reports include the fatty acid profiles, a listing of the best database matches, along with an assigned statistical probability value indicating the confidence level of the identifications.
Fungal BIOLOG®
This automated, identification system uses a 96-well microtiter plate with 95 different carbon sources. The microorganism of interest is applied to each well. Each microorganism has unique capacities to oxidize some of the various carbon sources. When these carbon sources are oxidized by the microorganism, a purple dye develops visible patterns of positive (purple) and negative (clear) wells which provide a metabolic signature of the organism. The system`s computer examines the pattern signature with its database to determine an identification. Databases include aerobic and anaerobic bacteria, yeasts and fungi.
Biofeasibility Studies
One of the most frequently requested set of tests is the Bioremediation Feasibility Series. It is a straight forward series of analyses that provide an objective evaluation of a site for bioremediation. The flow-chart shows the progression of tests as they are performed.
The Sample can be soil, water, or sludge. When the sample is received at the laboratory, it is immediately prepared for the Total Heterotrophic Plate Count. Over the next 48 hours the Petri plates containing dilutions of the samples are incubated for optimum bacteria growth. The populations are counted twice, once at 24 hours and again at 48 hours. At the end of 48 hours, each bacterium has grown into a colony. Each colony is distinguishable by size, color and texture. It is at this time we determine, by colony morphology, the number of different types of bacteria in the soil or water. The next step is Isolation. Each type of bacterial colony is picked, using a sterile loop, and then streaked onto a new Petri plate. These plates are then incubated for 24 hours in order to produce sufficient quantities of the strain. The isolated strains of bacteria are then harvested for processing in the Endpoint Assay and for identification by GC-FAME and Biolog™.
The Endpoint Assay tests the individual bacterial strains for their ability to use a specific substrate/contaminant as its only carbon source. This custom designed test uses a negative control of water and a positive control of a nutrient broth. The growth success of the bacteria is illustrated in a bar chart report. The Identification of the bacterial strains takes place while the Endpoint assay is running. Two methods are employed as a cross-check for precision and to provide an exact profile of the strain. The GC-FAME method provides a profile of the fatty acid composition of the bacteria and the Biolog™ method provides a characterization of the bacteria by the carbon sources the strain can consume. Next, the raw data is compiled and a summary Table of the bacterial identifications and Bar Charts of the Endpoint Assay raw data are created. These two items are incorporated in the full Report at the conclusion of all tests.
The Bioremediation Feasibility Study is complete at this point. If either the client or the MiL provides nutrient Chemistry Results, we will integrate the results within the Bioremediation Feasibility Study in order to provide an Estimated Project Length report. This report estimates the time it would take your site to reach specified clean-up goals under ideal conditions.
Other services include
Environmental Microbiology Services
The MiL, Inc.`s environmental lab, is an independent, full-service microbiology laboratory, providing a broad range of microbial and biochemical analytical services targeted to the environmental industry. Our laboratories are staffed with skilled and internationally recognized Ph.D. microbiologists and Ph.D. biochemists. Lab services include the isolation, enumeration, identification and R&D analyses of environmental microorganisms using leading edge technologies. Consulting services are focused towards method development and validation, bioremediation feasibility studies and testing for soils and wastewaters, bacterial genetics, soil nutritional analysis, specialized chemistries, and regulatory issues. Our diversified and worldwide customer base includes Fortune 500 companies, environmental engineering and consulting firms, dedicated bioremediation service providers, manufacturers and distributors of microbial cultures, marketers of nutrients and surfactants, research groups, and local, state and federal regulatory entities including many national laboratories. Bacteria and their enzymes, along with some fungi and critical nutrient additives are cost effective agents for in-situ remediation of hazardous wastes and subsurface pollution in soils, sediments and wastewaters. The ability of each bacteria strain to degrade toxic waste depends on the nature of each contaminant. Since most sites are typically comprised of multiple pollutant types, the most effective approach to bioremediation is to use a mixture of bacterial species/strains, each specific to the degradation of one or more types of contaminants. It is critical to monitor the composition of the indigenous and added bacterial consortium in order to evaluate the activity level of the bacteria, and to permit modifications of the nutrients and other conditions for optimizing the bioremediation process. High end, analytical microbiology and chemistry lab instrumentation and trained scientific staff are provided by the MiL to effectively monitor the bacteria population activity, nutrient chemistries and ambient conditions during all optimized bioremediation phases. At the MiL, Inc., our scientists developed the 48-hour, Endpoint Assay Bioremediation Feasibility Test. This test predicts site closure date estimates and is offered in response to the need for a faster and more precise way to evaluate bioremediation as a method of restoring contaminated land and groundwater. We were the first service laboratory in the United States to offer both Gas Chromatography Fatty Acid Methyl Ester (GC-FAME) and Biolog analyses. Together, these analyses along with our DNA sequence identifications and quantitative real-time PCR assays are the most accurate techniques to identify bacteria from environmental sources. The GC-FAME method with our extensive databases, identifies individual bacteria species down to the strain level, based on the fatty acid content of their cell walls. The Biolog method provides a characterization of the bacteria species according to how efficiently the microbe consumes targeted carbon sources. Rapid, computerized GC FAME and Biolog identifications and confirmations are available within 24 hours of receipt of samples. Our extensive microorganism databases, include aerobic and anaerobic bacteria strains, actinomycetes, yeasts, molds, and other fungi, ensures the most timely and accurate identifications available. We identify and quantitate populations of chlorohydrocarbon degraders including Dehalococoides species by qPCR. The MiL, Inc. is USDA recognized and has the permits to receive soil and groundwater samples from foreign countries and restricted areas in the U.S. Send Fedex samples in a cooler for rapid same day testing. We are also registered as a select agent laboratory for anthrax research. Specific services include Biofeasibility Studies Characterization of Biodegrading Microorganisms Bioremediation Monitoring Microcosm Treatability Testing Wastewater Biotreatment Consulting Services
The MiL, Inc.`s environmental lab, is an independent, full-service microbiology laboratory, providing a broad range of microbial and biochemical analytical services targeted to the environmental industry. Our laboratories are staffed with skilled and internationally recognized Ph.D. microbiologists and Ph.D. biochemists. Lab services include the isolation, enumeration, identification and R&D analyses of environmental microorganisms using leading edge technologies. Consulting services are focused towards method development and validation, bioremediation feasibility studies and testing for soils and wastewaters, bacterial genetics, soil nutritional analysis, specialized chemistries, and regulatory issues. Our diversified and worldwide customer base includes Fortune 500 companies, environmental engineering and consulting firms, dedicated bioremediation service providers, manufacturers and distributors of microbial cultures, marketers of nutrients and surfactants, research groups, and local, state and federal regulatory entities including many national laboratories.
Bacteria and their enzymes, along with some fungi and critical nutrient additives are cost effective agents for in-situ remediation of hazardous wastes and subsurface pollution in soils, sediments and wastewaters. The ability of each bacteria strain to degrade toxic waste depends on the nature of each contaminant. Since most sites are typically comprised of multiple pollutant types, the most effective approach to bioremediation is to use a mixture of bacterial species/strains, each specific to the degradation of one or more types of contaminants. It is critical to monitor the composition of the indigenous and added bacterial consortium in order to evaluate the activity level of the bacteria, and to permit modifications of the nutrients and other conditions for optimizing the bioremediation process. High end, analytical microbiology and chemistry lab instrumentation and trained scientific staff are provided by the MiL to effectively monitor the bacteria population activity, nutrient chemistries and ambient conditions during all optimized bioremediation phases.
At the MiL, Inc., our scientists developed the 48-hour, Endpoint Assay Bioremediation Feasibility Test. This test predicts site closure date estimates and is offered in response to the need for a faster and more precise way to evaluate bioremediation as a method of restoring contaminated land and groundwater. We were the first service laboratory in the United States to offer both Gas Chromatography Fatty Acid Methyl Ester (GC-FAME) and Biolog analyses. Together, these analyses along with our DNA sequence identifications and quantitative real-time PCR assays are the most accurate techniques to identify bacteria from environmental sources. The GC-FAME method with our extensive databases, identifies individual bacteria species down to the strain level, based on the fatty acid content of their cell walls. The Biolog method provides a characterization of the bacteria species according to how efficiently the microbe consumes targeted carbon sources. Rapid, computerized GC FAME and Biolog identifications and confirmations are available within 24 hours of receipt of samples. Our extensive microorganism databases, include aerobic and anaerobic bacteria strains, actinomycetes, yeasts, molds, and other fungi, ensures the most timely and accurate identifications available. We identify and quantitate populations of chlorohydrocarbon degraders including Dehalococoides species by qPCR. The MiL, Inc. is USDA recognized and has the permits to receive soil and groundwater samples from foreign countries and restricted areas in the U.S. Send Fedex samples in a cooler for rapid same day testing. We are also registered as a select agent laboratory for anthrax research.
Specific services include
Food Microbiology Services
Test Services Include Isolation, enumeration, and identification of pathogenic, indicator and spoilage organisms; challenge and shelf life studies; sanitation audits; QA and HACCP consultation, and expert witness work are a few of our services in the realm of food microbiology. We are equipped to test food and food-grade products as well as environmental surfaces and ambient air within food production facilities. Our specialties include microbial identification and confirmations, R and D analyses, consulting, method development and validations, toxins, challenge and shelf life studies, sanitation audits, QA and HACCP consultations. Overnight your food samples and isolates in our convenient sample cooler kit, for rapid overnight testing of pathogens such as Salmonella, Listeria, Ecolab O157:H7, Campylobacter, Shield and Staphylococcus aurous. Food Pathogen Testing Some of the species we routinely test: Bacillus cereus, Campylobacter, Clostridium outline, Clostridium porringers, Hemorrhagic and Enter pathogenic E. coli, Wisteria monocytogenes, Salmonella, Shigella, Staphylococcus, Vibro species, Yersinia enterocolitica Quantitative Indicator and Spoilage Testing - * Aerobic and Anaerobic plate counts * Coliforms * E. coli * Enterococci * Yeast and Mold counts * Acidophiles * Psychrotrophs * Spore counts * Thermophiles Microbial Characterization Technology Available - see Microbial Identification Methods * ELISA * DNA probes * 3M Petrifilm * Gas Chromatography Fatty Acid Analysis (GC-FAME) * BiologTM * RiboPrinterTM (QualiconTM) * MicroSeqTM 16S rRNA Gene Sequencing - 500 or 1540 base pair (PE Applied Biosystems) * qPCR Other Related Test Services The Mil also conducts other food related analyses such as Detection of Microbial Toxins (Aflatoxin, Botulinum toxin, Staphylococcus Enterotoxin, Vomitoxin, and other mycotoxins)Metabolites (such as histamine), antibiotics, inhibitory substances, food nutritional and vitamin chemistry, Meat Speciation (up to eleven species of meat differentiation), Commercial sterility, edible oil fatty acid profiles, carbohydrates, fats, fiber, ash, moisture, acridine orange direct counts, dark field /bright field/ epi-fluorescence photomicroscopy. More services Food Quality Testing Meat Adulteration ELISA Testing HAACP Consultation Services
Test Services Include
Isolation, enumeration, and identification of pathogenic, indicator and spoilage organisms; challenge and shelf life studies; sanitation audits; QA and HACCP consultation, and expert witness work are a few of our services in the realm of food microbiology.
We are equipped to test food and food-grade products as well as environmental surfaces and ambient air within food production facilities. Our specialties include microbial identification and confirmations, R and D analyses, consulting, method development and validations, toxins, challenge and shelf life studies, sanitation audits, QA and HACCP consultations. Overnight your food samples and isolates in our convenient sample cooler kit, for rapid overnight testing of pathogens such as Salmonella, Listeria, Ecolab O157:H7, Campylobacter, Shield and Staphylococcus aurous.
Food Pathogen Testing
Some of the species we routinely test: Bacillus cereus, Campylobacter, Clostridium outline, Clostridium porringers, Hemorrhagic and Enter pathogenic E. coli, Wisteria monocytogenes, Salmonella, Shigella, Staphylococcus, Vibro species, Yersinia enterocolitica
Quantitative Indicator and Spoilage Testing - * Aerobic and Anaerobic plate counts * Coliforms * E. coli * Enterococci * Yeast and Mold counts * Acidophiles * Psychrotrophs * Spore counts * Thermophiles
Microbial Characterization Technology Available - see Microbial Identification Methods * ELISA * DNA probes * 3M Petrifilm * Gas Chromatography Fatty Acid Analysis (GC-FAME) * BiologTM * RiboPrinterTM (QualiconTM) * MicroSeqTM 16S rRNA Gene Sequencing - 500 or 1540 base pair (PE Applied Biosystems) * qPCR
Other Related Test Services
The Mil also conducts other food related analyses such as
Detection of Microbial Toxins (Aflatoxin, Botulinum toxin, Staphylococcus Enterotoxin, Vomitoxin, and other mycotoxins)Metabolites (such as histamine), antibiotics, inhibitory substances, food nutritional and vitamin chemistry, Meat Speciation (up to eleven species of meat differentiation), Commercial sterility, edible oil fatty acid profiles, carbohydrates, fats, fiber, ash, moisture, acridine orange direct counts, dark field /bright field/ epi-fluorescence photomicroscopy.
More services
Microbial Identification Methods
GC-FAME (Gas Chromatograph Fatty Acid Methyl Ester) Also known as Cellular Fatty Acid Analysis, allows for bacterial or yeast identification based on the specific fatty acid composition of the cell wall. Fatty acids are extracted from cultured samples and are separated by gas chromatography. A computer generated, unique profile pattern of the extracted fatty acids (9 to 20 carbon chains long) is compared through pattern recognition programs, to our microbial databases of well over 2800 species and subspecies. Our reports include the fatty acid profiles, a listing of the best database matches, along with an assigned statistical probability value indicating the confidence level of the match. Biolog® MicroLog This automated metabolic characterization system uses a 96-well microtiter plate arrayed with different carbon sources and the microorganism of interest. Each microorganism has unique capacities to oxidize the various carbon sources. Tetrazolium dye in each of the wells turn darker shades of purple as the carbon sources are oxidized by the microorganism. The test yields a characteristic pattern of positive (purple) and negative wells which provide a metabolic signature of the inoculated organism. The system`s computer the pattern signature with the database of interest to determine an identification. Databases include aerobes, anaerobes, gram positive & negative bacteria, yeast, actinomycetes, and lactic acid bacteria. RiboPrinter™ (Qualicon™, a DuPont subsidiary) The RiboPrinter™ system characterizes bacteria by creating a fingerprint pattern of an isolate`s genetic material. Pure colonies are isolated from culture, inactivated by heat and loaded into the sample carrier. DNA is extracted from the cell lysate and is digested to completion using an EcoR1 restriction enzyme. The DNA fragments are separated according to molecular size by gel electrophoresis and are the transferred to moving Nylon membranes. After denaturation, each membrane is hybridized with a chemically labeled rRNA operon probe from E. coli. The membrane is washed and treated with a blocking buffer and an antisulfonated, DNA antibody/alkaline phosphatase conjugate. Unbound conjugate is removed and a chemiluminescent substrate applied. This step makes each electrophoresis band containing genetic information from the rRNA genes visible to a custom CCD camera in the RiboPrinter™ system. The camera detects the light intensity of the targeted bands and converts the patterns from luminescent DNA fragments to digital information. Using algorithms, a pattern is extracted from the image data and is compared to other known patterns stored in the database to characterize and identify each sample. MicroSeq™ 16S rRNA Gene Sequencing (PE Applied Biosystems) Genomic DNA is extracted directly from dead or alive bacterial colonies grown under any conditions. The 16S rRNA gene is amplified using universal primers and thermalcyclers. The amplified 16S rRNA gene product is sequenced using dye terminator cycle sequencing chemistry. The sequence reactions are analyzed using automated DNA sequencers and software. Unknown bacterial samples are identified using the MicroSeq™ microbial identification software and 16S rRNA gene sequence database, containing over 1,500 entries. Dependant upon the level of confidence, speed and cost desired, either the entire 1540 base pair 16S rRNA gene, or a smaller portion of the gene can be (500 bp) sequenced. A printout of the actual sequence data and the identification is included in each report.
Also known as Cellular Fatty Acid Analysis, allows for bacterial or yeast identification based on the specific fatty acid composition of the cell wall. Fatty acids are extracted from cultured samples and are separated by gas chromatography. A computer generated, unique profile pattern of the extracted fatty acids (9 to 20 carbon chains long) is compared through pattern recognition programs, to our microbial databases of well over 2800 species and subspecies. Our reports include the fatty acid profiles, a listing of the best database matches, along with an assigned statistical probability value indicating the confidence level of the match.
Biolog® MicroLog
This automated metabolic characterization system uses a 96-well microtiter plate arrayed with different carbon sources and the microorganism of interest. Each microorganism has unique capacities to oxidize the various carbon sources. Tetrazolium dye in each of the wells turn darker shades of purple as the carbon sources are oxidized by the microorganism. The test yields a characteristic pattern of positive (purple) and negative wells which provide a metabolic signature of the inoculated organism. The system`s computer the pattern signature with the database of interest to determine an identification. Databases include aerobes, anaerobes, gram positive & negative bacteria, yeast, actinomycetes, and lactic acid bacteria.
RiboPrinter™ (Qualicon™, a DuPont subsidiary)
The RiboPrinter™ system characterizes bacteria by creating a fingerprint pattern of an isolate`s genetic material. Pure colonies are isolated from culture, inactivated by heat and loaded into the sample carrier. DNA is extracted from the cell lysate and is digested to completion using an EcoR1 restriction enzyme. The DNA fragments are separated according to molecular size by gel electrophoresis and are the transferred to moving Nylon membranes. After denaturation, each membrane is hybridized with a chemically labeled rRNA operon probe from E. coli. The membrane is washed and treated with a blocking buffer and an antisulfonated, DNA antibody/alkaline phosphatase conjugate. Unbound conjugate is removed and a chemiluminescent substrate applied. This step makes each electrophoresis band containing genetic information from the rRNA genes visible to a custom CCD camera in the RiboPrinter™ system. The camera detects the light intensity of the targeted bands and converts the patterns from luminescent DNA fragments to digital information. Using algorithms, a pattern is extracted from the image data and is compared to other known patterns stored in the database to characterize and identify each sample.
MicroSeq™ 16S rRNA Gene Sequencing (PE Applied Biosystems)
Genomic DNA is extracted directly from dead or alive bacterial colonies grown under any conditions. The 16S rRNA gene is amplified using universal primers and thermalcyclers. The amplified 16S rRNA gene product is sequenced using dye terminator cycle sequencing chemistry. The sequence reactions are analyzed using automated DNA sequencers and software. Unknown bacterial samples are identified using the MicroSeq™ microbial identification software and 16S rRNA gene sequence database, containing over 1,500 entries. Dependant upon the level of confidence, speed and cost desired, either the entire 1540 base pair 16S rRNA gene, or a smaller portion of the gene can be (500 bp) sequenced. A printout of the actual sequence data and the identification is included in each report.
Rapid Gluten Home Test Kit
Microbe Inotech Laboratories has a unique product called Rapid Gluten Home Test Kit. This Test Kit is handy and simple to use for all persons who are affected with gluten intolerance. Each package includes 5 test kits for $90 (price includes shipping in the US). How important is it to test your food for traces of gluten? If you have been diagnosed with gluten intolerance (also known as Celiac disease), this test kit is very valuable to purchase. Celiac disease is a genetic disorder that affects 1 in 133 Americans. In world wide level there are about 1 in 300 persons of Northern European origin are affected by gluten intolerance, but recent studies show that it also affects Hispanic, Black and Asian populations as well. Celiac disease is a permanent hypersensitivity to gluten proteins from flour and meal made from wheat, rye and barley that affects the small intestine. The classic symptoms range from diarrhea, weight loss and malnutrition. A Gluten-free diet is not always easy. For people who are affected by celiac disease must eliminate gluten from their diet. Complying with a gluten-free diet isn`t always easy especially when some cereals are added to manufactured foods as thickeners and are not always labeled properly. How can you take precautionary measures while eating at a restaurant? Each test kit is designed to fit in a purse or pocket, especially for people on the go, who would like to make sure they can simply test their food in a restaurant for safety precautions.
Microbe Inotech Laboratories has a unique product called Rapid Gluten Home Test Kit. This Test Kit is handy and simple to use for all persons who are affected with gluten intolerance.
Each package includes 5 test kits for $90 (price includes shipping in the US).
How important is it to test your food for traces of gluten? If you have been diagnosed with gluten intolerance (also known as Celiac disease), this test kit is very valuable to purchase.
Celiac disease is a genetic disorder that affects 1 in 133 Americans. In world wide level there are about 1 in 300 persons of Northern European origin are affected by gluten intolerance, but recent studies show that it also affects Hispanic, Black and Asian populations as well. Celiac disease is a permanent hypersensitivity to gluten proteins from flour and meal made from wheat, rye and barley that affects the small intestine. The classic symptoms range from diarrhea, weight loss and malnutrition.
A Gluten-free diet is not always easy. For people who are affected by celiac disease must eliminate gluten from their diet. Complying with a gluten-free diet isn`t always easy especially when some cereals are added to manufactured foods as thickeners and are not always labeled properly.
How can you take precautionary measures while eating at a restaurant? Each test kit is designed to fit in a purse or pocket, especially for people on the go, who would like to make sure they can simply test their food in a restaurant for safety precautions.
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