CATALINA BIOSOLUTIONS

Bioremediation Services

After 15 years in the bioremediation business, our customer satisfaction rate remains 100 percent. Most sites treated with BioCritters reach full closure on the first attempt, but Catalina BioSolutions will provide unlimited technical support throughout the project to ensure complete site closure. All of our products are environmentally safe and can be handled without concern. Bio Critters are Safety Level I and are non-pathogenic and non-opportunistic. You can access the Material Safety Data Sheets (MSDS) for all of Catalina BioSolutions` products from this website.

BioCritters comprises a consortium of naturally-occurring microbes found in soil and water throughout the world. All the microbes in BioCritters are found in the Berges Manual. The by-products of the bioremediation process are carbon dioxide, water and trace elements of fatty acids.

BioCritters are formulated to degrade specific contaminants and nothing else. The amount of BioCritters used is based on the estimation of the quantity of contaminant. Once the contaminant is cleaned, the BioCritters` food source is gone and they become inert. If there were another spill, the inert BioCritters would automatically revive. Warm weather will not affect the efficacy of BioCritters. Freezing temperatures rupture the cell walls of the microbes, although even freezing temperatures do not necessarily preclude a winter BioTreatment. Catalina BioSolutions has applied BioCritters in blizzards with excellent results by inoculating below the frost line where bioremediation can still occur.

Services

Catalina BioSolutions experience and expertise in the field are unparalleled. Unlimited technical support is included with all purchases of products and/or services, whether you are purchasing BioCritters and plan to do the work yourself, or you hire Catalina BioSolutions to clean the site. Besides product sales, Catalina BioSolutions offers the following services:

Feasibility Test

Free of charge, we will determine whether or not bioremediation is a feasible option for your particular contaminant. You will receive an honest and accurate assessment as to whether your site can be cleaned.

BioTreatability Study

The BioTreatability Study will furnish you with 3 points on a curve to help you determine the effectiveness and efficiency of Catalina BioSolutions BioTreatment System on your site. When you send us a small sample of the contaminant, we will inoculate the sample with 3 different blends of BioCritters, and then send the treated samples back to you at the 30-day and 60-day marks for your own lab analyses. (This way, you can feel confident that you are getting reliable results from your own lab.) The added advantage of Catalina BioSolutions` BioTreatability Study is that the cost of the study will be credited toward your purchase of product and/or services. Please call for BioTreatability Study costs.

Inoculation Services

Catalina BioSolutions` Inoculation Services include mixing the BioCritters on site, training your personnel as needed and performing one or more of the inoculations. This option does not include any site work.

Full Service Bioremediation

Catalina BioSolutions provides Full Service Bioremediation (in situ or ex situ) including: initial lab analyses; plate counts and lab tests throughout the project; inoculations and site work until the site reaches the required action levels; and the final lab analyses.

Photomicrography

Catalina BioSolutions can take digital photographs through a microscope with a 400X lense then e mail you the pictures of samples that you provide to us. This way you can actually see what you have. We can also print them out. This would be an excellent marketing tool for you.

BioTreatment Systems

BioTreatment System Includes

BioCritters

• Our special blends of naturally-occurring microbes grown in carbon pellets
• One pound treats 20 cubic yards of soil or 5,000 gallons of water 
• Comprises a variety of microorganisms which produce a broad spectrum of enzyme systems 
• Includes facultative anaerobes (to work with or without oxygen) and microflora 
• Can survive in the presence of heavy metals, salt and fresh water 
• Clean, compact, easy to measure 
• Doesn`t mold, mildew, develop mutations, or interest insects or animals 
• Safety level I
• Non-pathogenic 
• Non-opportunistic 
• Indefinite shelf life (if kept out of sunlight and freezing conditions) 
• Four times more microbes per pound

BioBooster

• Superior, slow-release nutrient source, stimulant, and accelerant 
• Produces excellent plate counts 
• Included with every purchase of BioCritters

FREE BioFeasability Test
FREE Unlimited Technical Support

Product shipped immediately
Upon receipt of payment

Biocritters Bio Treatment System for Organic and Inorganic Contaminants Cleaning

BioCritters degrade the following:

• All types of aliphatic and aromatic hydrocarbon degradation 
• Dichloroethylenes 
• Toluene 
• Dinitrotoluene 
• Crude Oils 
• Heptachlor 
• Phoenols 
• Dichlorethylenes 
• Naphthalene 
• Hydrocarbons 
• Nitrophenols 
• Benzene 
• Kerosene 
• Vinyl Chloride 
• Halomethanes 
• Gasoline 
• Diesel Fuel 
• MTBE 
• PCE 
• TCE

Free Biofeasibility Test

If you have an unusually challenging contaminant, such as PCB, send us a small sample. Free of charge, we will take this sample of the contaminant and determine whether or not bioremediation is a feasible option. You will receive an honest and accurate assessment as to whether our BioCritters can clean your site.

Carbon Biocritters

Material Safety Data Sheet for Carbon Biocritters

Section I - Company Identity

Catalina BioSolutions
6890 E. Sunrise Drive #120-110
Tucson, Arizona 85750
Emergency Phone: 520-299-9808

Section II - Hazardous Ingredients/Identity Information

Chemical Name & Synonyms: Activated carbon

Trade Name & Synonyms: Catalina BioSolutions` Tri-Based Carbon Pellets, Catalina BioSolutions` Carbon Biocritters

Formula: Carbon atom in a crystallite structure has an infinite molecular weight

Chemical Family: Amorphous carbon with three bases containing an enzymatic mixture of active hydrocarbon oxidizing natural single-celled organisms. The mixture has no chemical impact.

CAS No: 68647-86-9

Hazardous Ingredients: None

Hazardous Mixtures Other Liquids [%TLV (Units)]: Activated carbons that have adsorbed other carbon or non- carbon liquids or gasses may lower or raise the ignition point and must be laboratory checked for ignition point when expended

Section III - Physical Data

Boiling Point (°F): 4200°F
Vapor Pressure (mm/Hg): N/A
Vapor Density (Air=1): N/A
Solubility in Water: Insoluble
Specific Gravity (H20=1): 1.8–2.1
Percent Volatile by Volume: None
Evaporation Rate: None
Ignition Temperature: 600°C
Appearance & Odor: Odorless, black cylindrical solid

Section IV - Fire & Explosion Hazard Data

Flash Point (Method): None

Flammable Limits-
 Lower Explosive Limit: N/A
 Upper Explosive Limit: N/A
Extinguishing Media: Use media for Class A fires: foam, multipurpose dry chemical and water type extinguishers

Special Firefighting Procedures: None

Unusual Fire & Explosion Hazards: Provide for the handling of dry flowing solids in grounded equipment to prevent buildup of static electric charge, especially when explosive dust or vapor mixtures may exist in confined areas. Also provide for pressure relief devices following the principles set forth in the National Fire Protection Association Explosion Preventing Guide NFPS68-1854.

Section V - Health Hazard Data

Threshold Limit Value: Avoid exposure to dust levels above 15 mg per cubic meter

Effects of Overexposure: Temporary dryness to mucous membranes causing coughing and minor nose and throat irritation

Emergency First Aid Procedures: Wash mouth with water — no other treatment required. Use protective respiratory equipment to avoid inhaling carbon dust.

Section VI - Reactivity Data

Stability: Inert

Conditions to Avoid: Activated carbon is chemically inert

Incompatibility (Materials to Avoid): None

Hazardous Decomposition
Products: None

Hazardous Polymerization: Will not occur

Section VII - Spill or Leak Procedures

Steps to Be Taken in Case     Spills can create nuisance dust and housekeeping
Material is Released or Spilled: problems. Vacuuming is best clean-up method.

Waste Disposal Method: Wet or dry activated carbon is best disposed of by
        Landfill

Section VIII - Special Protection Information

Respiratory Protection: Respiratory Classification Table G-2, Part 1910.93 (OSHA)
(Specify Type):    Rules and Regulations

Ventilation Local Exhaust: Vacuum to control dust

Protective Gloves: None required

Eye Protection: For airborne dust

Other Protective Equipment: Protective clothing should be worn during handling to protect against airborne dust

Section IX - Special Precautions

Precautions To Be Taken    Packaged activated carbon is not resistant to weather or
In Handling & Storing: outside storage and requires indoor Type I and Type II storage facilities

Other Precautions:  Check oxygen content of atmosphere of any vessel containing activated carbon before allowing entry of personnel

Section X - Transportation Data

Proper Shipping (Article) Name: Catalina BioSolutions` Tri-Based Carbon Pellets/Non-Regulated; Catalina BioSolutions` Carbon Biocritters/Non-Regulated

DOT Classification: NMFC 40560
IDOT Marking: N/A
IDOT Placard: N/A

Emergency Accident Precautions & Procedures: Contact: Catalina BioSolutions
            Phone: 520-299-9808
Precautions to be taken in Transportation: N/A

The information contained herein is based on data considered accurate in light of current information. However, No warranty is expressed or implied regarding the accuracy of this data or the results to be obtained from the use thereof. This material safety sheet was prepared to comply with 24 CFR 1910.1200.

Bio Booster

Material Safety Data Sheet for Bio Booster

Section I - Company Identity

Catalina BioSolutions
6890 E. Sunrise Drive #120-110
Tucson, Arizona 85750
Emergency Phone: 520-299-9808

Section II - Hazardous Ingredients/Identity Information

Chemical Name & Synonyms: Confidential chemical blend

Trade Name & Synonyms: Catalina BioSolutions’
BioBooster;CatBioSolutions’SuperDuper Bacterial Booster

Product Uses: Bacterial food additive
Means of Classification: In-house

Molecular Weight: 148.01
WHIMS Classification: Not classified
PIN/UN No: N/A
CAS/PIN No: N/A
Test Related Info: N/A
Hazardous Ingredients: None
Hazard Ratings
(0=Least, 1=Slight, 2=Moderate,
3=High, 4=Extreme):
 Health 0
 Fire & Explosion 0
 Reactivity 0

Section III- Physical Data

Boiling Point (°F): N/A
Vapor Pressure (mm/Hg): N/A
Vapor Density (Air=1): N/A
Solubility in Water: 22g/100g @ 0°C
38g/100g @ 20°C
Percent Soluble (@ 20°C): N/A
Specific Gravity (H20=1): 1:8
Melting Point (°C): 180°C
Evaporation Rate: N/A
Coef. Water/Oil: N/A
Freezing Temp. (°F): 
Percent Volatile by Volume: N/A
pH: 6.1 (1% solution @ 20°C)
Appearance & Odor: Solid, white anhydrous crystals, odorless

Section IV - Fire & Explosion Hazard Data

Flash Point: N/A
Flammable Limits
  Lower Flammable Limit: N/A
  Upper Flammable Limit: N/A

Extinguishing Media: Water, fog, CO2, foam or dry chemical may be used
Special Firefighting Procedures: Wear NIOSH approved SCUBA when in a confined 
area or exposed to combustion products

Unusual Fire & Explosion Hazards: None known

Hazardous Combustion Products: Ammonia, phosphorous and nitrogen oxides are        .
released when heated. NOx may be released in a fire

Explosion
 Sensitivity to Impact: N/A
 Sensitivity to Static Discharge: N/A

Section V - Health Hazard Data

Carcinogenicity
 By NIP: N/A
 By IARC: N/A
 OSHA Regulated: N/A

Effects of Overexposure

• Acute
• Ingestion: May cause diarrhea, nausea, vomiting, cramps and burns to the
• mouth and throat if large amounts are ingested
• Inhalation: Dust and mist may be irritating to the nose and throat, causing
• coughing and choking
• Chronic: Dermatitis may develop from repeated or prolonged skin contact
   
  Exposure Limits
   TWA: N/A
   STEL: N/A
   C: N/A
   OSHA PEL: N/A
   ACGIH TLV: N/A
   Others: N/A
  Routes of Entry: Ingestion

Emergency First Aid Procedures
     Eyes: Flush thoroughly with water for 15 minutes while keeping upper and lower eyelids apart. Get medical attention.
     Skin: Remove contaminated clothing. Wash exposed areas with soap and water and rinse thoroughly for at least 15 minutes. Wash clothing before reuse. Get medical attention if irritation develops.
     Ingestion: Drink a glass of water or milk. DO NOT induce vomiting. Consult a physician immediately.
     Inhalation: Move victim to fresh air. Assist breathing, if necessary. Give oxygen or administer artificial respiration if required.
     Special Note: Never give fluids or induce vomiting to any unconscious victim. Place individual in stable position.

Irritants
     Eyes: May cause mild irritation
     Skin: May be irritating from prolonged skin contact. No effects if promptly rinsed off.

Sensitization: N/A
Terategenicity: N/A
Reproduction Toxicity: N/A
Mutagenicity: N/A
Synergistic Products: N/A
Lethality Tests: Value Related Information
     LD50 Oral N/A Tested on rats
     LD50 Dermal N/A Tested on rabbits
 LD50 Inhalation N/A Tested on rats

NOT FOR HUMAN CONSUMPTION

Section VI - Reactivity Data

Stability: Stable under normal conditions
Conditions to Avoid: Avoid excessive heat
Incompatibility (Materials to Avoid): Avoid contact with strong oxidizing agents and strong bases
Other Reactivity Concerns: Strong bases will release ammonia gas
Strong oxidizing agents can cause decomposition to occur

Section VII - Spill or Leak Procedures

Steps to be taken in Case Material is Released or Spilled: Wear necessary protective clothing and equipment. Sweep up while avoiding dust regeneration. Place in a properly labeled container for later disposal. Larger spills may require a vacuum.

Waste Disposal Method: May be deposited in a chemical dump. Disposal shall be in accordance to all applicable Federal and State waste regulations.

Section VIII - Special Protection Information

Respiratory Protection (Specify Type): Wear NIOSH/MSHA approved chemical cartage filter designed to remove dust particles during product application or if occupational exposure limits (TLV)

Ventilation Local Exhaust: General mechanical ventilation system is adequate
Protective Gloves: Rubber or plastic gloves
Eye Protection: Chemical goggles

Special: Remove and wash contaminated clothing before reuse. Wash thoroughly after handling.
Other Protective Equipment: None

Section IX- Special Precautions

Precautions to Be Taken in Handling & Storing: Do not handle unless the safety precautions have been read and understood. Avoid eye and skin contact. Avoid inhalation of dust or vapors. Do not puncture, drag, or slide containers. Do not smoke in any chemical handling or storage area. Wash hands before eating.

Storage Requirements: Store in a cool, dry and well-ventilated location. Avoid dust generation. Keep containers tightly closed. Store away from incompatible materials.

Section X - Transportation Data

Proper Shipping (Article) Name: Catalina BioSolutions’ BioBooster; Catalina BioSolutions’ Super Duper Bacterial Booster

Special Shipping Information: 49 CFR REGULATION (under HM-181 rule)
Not regulated for transport in the U.S.

Emergency Accident Precautions Contact: Catalina BioSolutions
& Procedures: Phone: 520-299-9808
 
The information contained herein is based on data considered accurate in light of current information. However, No warranty is expressed or implied regarding the accuracy of this data or the results to be obtained from the use thereof. This material safety sheet was prepared to comply with 24 CFR 1910.1200.

Case Studies

Groundwater Treatment of BTEX

Project: Treated 12,000 cubic yards of petroleum contaminated sandy-silty soil and groundwater
Client: Gate Petroleum
Location: South Central Florida
Scope of work: In-Situ Bioremediation
Project cost: $78,000 (Included only microbial products)
Source of contamination: Leaking UST
Project duration: 9 months
Description: A Geo-Probe was used to inject the microbial products into 100 borings. Two treatments have been performed since the underground storage tanks were upgraded.

In Situ Groundwater Treatment of Gasoline

Project: Treated 300 cubic yards of gasoline contaminated silty sands and groundwater
Client: Private Service Station Owner (Former Exxon station)
Location: Near Dayton, Ohio
Scope of work: In-Situ Bioremediation
Project cost: $7,575 (Included earthmoving, labor, and all bioremediation materials)
Source of contamination: Leaking UST
Project duration: 3 weeks
Status: Completed
Description: An open pit was excavated as a means to inoculate the contaminated groundwater. A liquid mixture of our microbes and nutrients was pumped into the pit and then agitated by the backhoe. Two treatments were performed during a two-week time period. Three weeks after the initial treatment, contamination levels dropped below action levels and the project was closed.

Free Product (Diesel) in Groundwater

Project: Treated 2,500 cubic yards of diesel in groundwater
Client: Mission Bay Resort Hotel
Location: San Diego, California
Scope of work: In-Situ Bioremediation
Project cost: $175,000 (Included labor and materials for treating groundwater 4 times)
Source of contamination: Leaking UST
Project duration: 12 months
Status: Completed
Description: The plume for this site was located below the parking garage for a resort hotel in southern California. Injection wells were installed and used as a means for application of the microbes into the soil. In addition to using our own consortium of microbes and nutrients, we added a bio-catalyst on this project to provide additional oxygen for the microbes. Twelve months after the initial treatment, the TPH dropped from 130,000 ppm to less than 1 ppm.

In Situ / Ex Situ Soil Treatment of Diesel

Project: Treated 100 cubic yards of diesel contaminated sand and silts
Client: Private Service Station Owner (Gambler Service Station)
Location: Near Gary, Indiana
Scope of work: In-Situ Bioremediation
Project cost: $1,400 (Included labor and materials for treating soil once)
Source of contamination: Leaking UST
Project duration: 4 months
Status: Completed
Description: An area of 25' x 25' x 5' was excavated, treated with microbes and nutrients, and then placed back into the pit. A small, rubber tire back-hoe was used for the earthmoving during this project.

Ex Situ Soil Treatment of Waste Oils and Diesel Fuel

Project: Treated 450 cubic yards of waste oils and diesel fuel in soil and rock
Client: Southern Arizona Mining Site
Location: Near Tucson, Arizona
Scope of work: Ex-Situ Bioremediation
Project cost: $21,600 (Included earthmoving equipment, treatment, bioremediation supplies and all analytical sampling)
Source of contamination: Leaking USTs and mining equipment
Project duration: 6 weeks
Status: Completed (Catalina BioSolutions treats a new cell of 200-400 cubic yards of soil every 4-8 weeks)
Description: A treatment cell has been constructed to hold 250-500 cubic yards of soil. Approximately once a month, Catalina BioSolutions turns the 18-22" high soil in the Bio-Cell and sprays the soil down with the liquid microbial product.

Soil and Groundwater Treatment of PCE

Project: Treated 100 Cubic Yards of PCE Contaminated Clay Soil and Groundwater
Client: Former Dry Cleaners
Location: Warren, Michigan
Scope of work: In-Situ Bioremediation
Source of contamination: Leaking UST
Project duration: 2 months
Status: Treatment Completed
Description: A series of 2 inch wide injection wells were drilled to a depth of approximately 10 feet. Our bioremediation products were injected in liquid form into each well. One performance well was left untreated in the center of the source area. The analytical results above are from that performance well.

Soil Treatment of Chlordane

Project: Ex-Situ Pilot Test; Silt and Clay Soils
Client: Former Pesticide Manufacturing Plant
Location: Eastern North Carolina
Scope of work: Ex-Situ Bioremediation
Source of contamination: Several Years of Surface Spills
Project duration: 4 months

Soil & Groundwater Treatment of Tetrochloroethene (PERC)

Project: Treated 200-300 cubic yards of Tetrachloroethene contaminated soil and groundwater
Client: Dry Cleaners
Location: Lexington, Kentucky
Scope of work: In-Situ Bioremediation
Project cost: $3,500 per treatment (Included products and labor)
Source of contamination: Leaking UST
Project duration: 1 Year
Description: A microbial solution was injected into a series of 2 inch treatment wells. The cleanup target levels for this site are 5 parts per billion of Tetrachloroethene.

Soil and Groundwater Treatment of MTBE

Project: Treated 4,200 cubic yards of petroleum contaminated sandy-silty soil and water
Client: Speedy Express Service Station
Location: Walterboro, South Carolina
Scope of work: In-Situ Bioremediation
Project cost: $30,000 (Included only microbial products)
Source of contamination: Leaking UST
Project duration: 15 months
Description: A series of 2-inch diameter slotted PVC was installed to a depth of 8-10 feet. Approximately 200 gallons of our microbial and nutrient product was injected into each well: three times over a period of nine months.

Microbial Solutions

Mixing Instructions for Microbial Solutions

Attention: Once You Begin The Mixing Process, Use The Microbial Solution Within 24-36 Hours.

Product:

• Dechlorinator (included with purchase of BioCritters)
• Nylon Mesh Bag (included with purchase of BioCritters)
• 4 cups BioCritters per 55-gallons water
• 2 cups BioBooster per 55-gallons water

Ideal Conditions:

• Water Temperature of 83º F.
• pH of 7-8
• Dissolved Oxygen (DO) level of 2-3 ppm

Equipment:

• Clean, standard 55-gallon drum (If you are using a larger tank, the proportions remain the same - 4 cups of BioCritters and 2 cups of BioBooster for every 55-gallons of water.)
• 300-gallons-per-hour or a 1,200 gph submersible pump, optional. (Sometimes there is no access to electricity in the field. While using the pump is ideal, it is not imperative. BioCritters will work fine without it.)

The pump mixes the solution and generates heat, which provides the near-ideal temperature of 83º F. We recommend either the 300 gph or the 1,200 gph submersible pumps manufactured by Little Giant. These and similar products are readily found at hardware stores. You should be able to buy a quality pump for under $100.

• PVC pipe cut to run from the discharge end of the pump to 6-8 inches above water level (The water shooting out will create a small fountain effect, which aerates the mixture.)

Preparing the Microbes:

1. Put 50 gallons of water into the 55-gallon drum.
2. Add the dechlorinator.
3. Attach the PVC pipe to the discharge outlet in the pump.
4. Submerge the pump and turn it on.
5. Add 2 cups BioBooster to the water.
6. Put 4 cups BioCritters into the mesh nylon bag.
7. Put the nylon mesh bag of BioCritters into the water.

The BioCritters solution will be ready when the mixture looks milky and translucent. Also, the solution will smell pungently sweet. There may be a lot of froth. The same carbon BioCritters can be used again for your second inoculation. After mixing up your first batch of BioCritters, store the nylon mesh bag of BioCritters in a dry place.

For the Second Inoculation:

For the second inoculation, repeat the process, using the same nylon mesh bag of BioCritters you used for the first inoculation. Remember to add 4 cups of BioBooster to the water. Two inoculations, 30-40 days apart, are ideal. After mixing your second batch of BioCritters, discard the bag of product.